Automated 5′ Nuclease PCR Assay for Identification of Salmonella enterica
نویسندگان
چکیده
منابع مشابه
Modeling of 5' nuclease real-time responses for optimization of a high-throughput enrichment PCR procedure for Salmonella enterica.
The performance of a 5' nuclease real-time PCR assay was studied to optimize an automated method of detection of preenriched Salmonella enterica cells in buffered peptone water (BPW). The concentrations and interactions of the PCR reagents were evaluated on the basis of two detection responses, the threshold cycle (C(T)) and the fluorescence intensity by a normalized reporter value (DeltaR(n))....
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2000
ISSN: 1098-660X,0095-1137
DOI: 10.1128/jcm.38.9.3429-3435.2000